Sains Malaysiana 53(4)(2024): 881-892

http://doi.org/10.17576/jsm-2024-5304-12

 

Evaluating the Cytotoxic Activity of Lactobacillus plantarum IIA-1A5 against MCF-7 Human Breast Cancer Cells and Identifying Its Surface Layer Protein Gene

(Menilai Aktiviti Sitotoksik Lactobacillus plantarum IIA-1A5 terhadap Sel Kanser Payudara Manusia MCF-7 dan Mengenal Pasti Gen Protein Lapisan Permukaannya)

 

REZA ADIYOGA1, CAHYO BUDIMAN1, ZAENAL ABIDIN2, KAZUHITO FUJIYAMA3& IRMA ISNAFIA ARIEF1,*

 

1Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia

3International Center for Biotechnology, Osaka University, Yamada-oka 2-1, Suita, Osaka 565-0871, Japan

 

Received: 4 June 2023/Accepted: 28 February 2024

 

Abstract

Breast cancer is a serious global health concern, with a high mortality rate worldwide. Using natural compounds as potential cancer therapies is a promising approach to address this issue. Previous research has shown that probiotic lactic acid bacteria (LAB) and their metabolites, such as surface layer protein (slp), have a positive impact on a variety of health disorders, including cancer. The purpose of this study was to evaluate the ability of Lactobacillus plantarum IIA-1A5 to suppress the growth of the MCF-7 breast cancer cell line and detect the presence of the slp gene. Intracellular and extracellular protein fractions were isolated from L. plantarum IIA-1A5 cultures. The protein concentrations and molecular weights of the extracts were measured. The anticancer activity of the extracts was assessed using the MTT cytotoxicity test, and IC50 values were calculated. The slp gene was identified through polymerase chain reaction (PCR) amplification and nucleotide sequencing. The results demonstrated that L. plantarum IIA-1A5 had a concentration-dependent inhibitory effect on MCF-7 breast cancer cells, with IC50 values of 6.831 and 12.35 μg/mL for intracellular extracts and extracellular extracts, respectively. Additionally, PCR amplification and nucleotide sequencing confirmed the presence of the slp gene, which may contribute to the strain’s anticancer abilities. These findings suggest the potential of L. plantarum IIA-1A5 as a natural anticancer agent against MCF-7 breast cancer cells. Further research is warranted to elucidate the underlying mechanisms of L. plantarum IIA-1A5 in breast cancer treatment.

 

Keywords: Anticancer; breast cancer; lactic acid bacteria; MCF-7; slp gene

 

Abstrak

Kanser payudara merupakan isu kesihatan global utama dengan kadar kematian yang tinggi di seluruh dunia. Penggunaan sebatian semula jadi sebagai terapi kanser merupakan strategi yang berpotensi untuk menangani isu ini. Kajian terdahulu telah mencadangkan bahawa bakteria asid laktik probiotik (LAB) dan metabolitnya seperti protein lapisan permukaan (slp) telah menunjukkan kesan yang baik terhadap pelbagai keadaan kesihatan, termasuk kanser. Kajian ini bertujuan untuk menyelidik potensi Lactobacillus plantarum IIA-1A5 dalam menghalang pertumbuhan sel kanser payudara MCF-7 dan mengenal pasti kehadiran gen slp. Fraksi protein intrasel dan ekstrasel diekstrak daripada kultur L. plantarum IIA-1A5. Konsentrasi protein dan berat molekul ekstrak dianalisis. Aktiviti antikanser ekstrak dinilai menggunakan ujian sitotoksik MTT dan nilai IC50 ditentukan. Amplifikasi rantai polimerase (PCR) dan penjujukan nukleotida dilakukan untuk mengenal pasti gen slp. Hasil kajian menunjukkan bahawa L. plantarum IIA-1A5 menunjukkan kesan perencatan yang bergantung kepada kepekatan ke atas sel kanser payudara MCF-7, dengan nilai IC50 6.831 μg/mL untuk ekstrak intrasel dan 12.35 μg/mL untuk ekstrak ekstrasel. Kehadiran gen slp disahkan dalam strain tersebut melalui amplifikasi PCR dan penjujukan nukleotida. Penemuan ini menunjukkan potensi L. plantarum IIA-1A5 sebagai agen antikanser semula jadi untuk rawatan kanser payudara MCF-7. Gen slp yang dikenal pasti mungkin menyumbang kepada aktiviti antikanser strain tersebut. Penyelidikan lanjut diperlukan untuk memahami mekanisme asas L. plantarum IIA-1A5 dalam rawatan kanser payudara.

 

Kata kunci: Antikanser; bakteria asid laktik; gen slp; kanser payudara; MCF-7

 

REFERENCES

Adiyoga, R., Arief, I.I., Budiman, C. & Abidin, Z. 2022. In vitro anticancer potentials of Lactobacillus plantarum IIA-1A5 and Lactobacillus acidophilus IIA-2B4 extracts against WiDr human colon cancer cell line. Food Sci. Technol. 42: e87221.

Alp, D., Kuleaşan, H. & Altıntaş, A.K. 2020. The importance of the S-layer on the adhesion and aggregation ability of lactic acid bacteria. Mol. Biol. Rep. 47: 3449-3457.

Aragón, F., Carino, S., Perdigón, G. & de Moreno de LeBlanc, A. 2015. Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431. Journal of Immunotherapy 38(5): 185-196.

Arief, I.I., Jenie, B.S.L., Astawan, M., Fujiyama, K. & Witarto, A.B. 2015. Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef. Asian J. Anim. Sci. 9: 25-36.

Arief, I.I., Jakaria, Suryati, T., Wulandari, Z. & Andreas, E. 2013. Isolation and characterization of plantaricin produced by Lactobacillus plantarum strains (IIA-1A5, IIA-1B1, IIA-2B2). Media Peternakan 36: 91-100.

 Atjanasuppat, K., Wongkham, W., Meepowpan, P., Kittakoop, P., Sobhon, P., Bartlett, A. & Whitfield, P.J. 2009. In vitro screening for anthelmintic and antitumour activity of ethnomedicinal plants from Thailand. J. Ethnopharmacol. 123: 475-482.

Aykul, S. & Martinez-Hackert, E. 2016. Determination of half-maximal inhibitory concentration using biosensor-based protein interaction analysis. Anal. Biochem. 508: 97-103.

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72: 248-254.

Birnboim, H.C. & Doly, J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7: 1513-1523.

Boot, H.J., Kolen, C.P.A.M., van Noot, J.M. & Pouwels, P.H. 1993. S-layer proteins of Lactobacillus acidophilus ATCC 4356: purification, expression in Escherichia coli and nucleotide sequence of the corresponding gene. Journal of Bacteriology. 175: 6089-6096.

Cao, J., Zhang, M., Wang, B., Zhang, L., Fang, M. & Zhou, F. 2021. Chemoresistance and metastasis in breast cancer molecular mechanisms and novel clinical strategies. Front. Oncol. 11: 658552.

Chen, X., Xu, J., Shuai, J., Chen, J., Zhang, Z. & Fang, W. 2007. The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157: H7 and Salmonella typhimurium. Int. J. Food Microbiol. 115: 307-312.

Dennert, G. & Horneber, M. 2006. Selenium for alleviating the side effects of chemotherapy, radiotherapy and surgery in cancer patients. Cochrane Database Syst. Rev. 2006(3): CD005037.

Ding, C., Tang, W., Fan, X. & Wu, G. 2018. Intestinal microbiota: A novel perspective in colorectal cancer biotherapeutics. OncoTargets and Therapy 11: 4797-4810.

Dolati, M., Tafvizi, F., Salehipour, M., Movahed, T.K. & Jafari, P. 2021. Inhibitory effects of probiotic Bacillus coagulans against MCF7 breast cancer cells. Iran J. Microbiol. 13(6): 839-847.

Ford, M.J., Normellini, J.F. & Smit, J. 2007. S-layer anchoring and localization of an S-layer-associated protease in Caulobacter crescentus. Journal of Bacteriology 189(6): 2226-2237.

Gupta, R., Jeevaratnam, K. & Fatima, A. 2018. Lactic acid bacteria: Probiotic characteristic, selection criteria, and its role in human health (A review). Journal of Emerging Technologies and Innovative Research 5(10): 411-424.

Harbeck, N., Penault-Llorca, F., Cortes, J., Gnant, M., Houssami, N., Poortmans, P., Ruddy, K., Tsang, J. & Cardoso, F. 2019. Breast cancer. Nat. Rev. Dis. Primers 5: 66.

Laemmli, U.K. 1970. Cleavage of the structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685.

Li, P., Yin, Y., Yu, Q. & Yang, Q. 2011. Lactobacillus acidophilus S-layer protein-mediated inhibition of Salmonella-induced apoptosis in Caco-2 cells. Biochem. Biophys. Res. Commun. 409(1): 142-147.

Liu, C.F. & Pan, T.M. 2010. In vitro effects of lactic acid bacteria on cancer cell viability and antioxidant activity. Journal of Food and Drug Analysis 18(2): 77-86.

Liu, C., Zheng, J., Ou, X. & Han, Y. 2021. Anticancer substances and safety of lactic acid bacteria in clinical treatment. Frontiers in Microbiology 12: 722052.

Luqmani, Y.A. 2005. Mechanisms of drug resistance in cancer chemotherapy. Med. Princ. Pract. 14: 35-48.

Mathur, H., Beresford, T.P. & Cotter, P.D. 2020. Health benefits of lactic acid bacteria (LAB) fermentates. Nutrients 12(6): 1679.

Meng, J., Zhang, Q.X. & Lu, R.R. 2018. Identification and analysis of the function of surface layer proteins from three Lactobacillus strains. Ann. Microbiol. 68: 207-216.

Meng, J., Zhu, X., Gao, S.M., Zhang, Q.X., Sun, Z. & Lu, R.R. 2014. Characterization of surface layer proteins and its role in probiotic properties of three Lactobacillus strains. Int. J. Biol. Macromol. 65: 110-114.

Ningtiyas, W.D., Arief, I.I., Budiman, C. & Utomo, A.R.H. 2021. Inhibition of human cervical cancer Hela cell line by meat-derived lactic acid bacteria of Lactobacillus plantarum IIA-1A5 and Lactobacillus acidophilus IIA-2B4. Pak. J. Biol. Sci. 24: 1340-1349.

Nogueira, T., Touchon, M. & Rocha, E.P.C. 2012. Rapid evolution of the sequences and gene repertoires of secreted proteins in bacteria. PLoS ONE 7(11): e49403.

Nowak, A., Paliwoda, A. & Blasiak, J. 2019. Anti-proliferative, pro-apoptotic and anti-oxidative activity of Lactobacillus and Bifidobacterium strains: A review of mechanisms and therapeutic perspectives. Crit. Rev. Food Sci. Nutr. 59: 3456-3467.

Nowroozi, J., Mirzaii, M. & Norouzi, M. 2004. Study of Lactobacillus as probiotic bacteria. Iranian J. Publ. Health. 33: 1-7.

Oskoueian, E., Abdullah, N., Saad, W.Z., Omar, A.R., Kuan, W.B., Zolkifli, N.A., Hendra, R. & Ho, Y.W. 2011. Antioxidant, anti-inflammatory and anticancer activities of methanolic extracts from Jatropha curcas Linn. J. Med. Plants Res. 5: 49-57.

Paiva, A.D., Oliveira, M.D., Paula, S.O., Baracat-Pereira, M.C., Breukink, E. & Mantovani, H.C. 2012. Toxicity of bovicin HC5 against mammalian cell lines and the role of cholesterol in bacteriocin activity. Microbiology 158(11): 2851-2858.

Ren, W., Qiao, Z., Wang, H., Zhu, L. & Zhang, L. 2003. Flavonoids: Promising anticancer agents. Medicinal Research Reviews 23: 519-534.

Sahlan, M., Azizah, N., Hakamada, K., Noguchi, K. & Yohda, M. 2018. Isolation and molecular weight characterization of Tetragonula laeviceps honey protein. Makara Journal of Technology 22(1): 9-12.

Sára, M. & Sleytr, U.B. 2000. S-layer proteins. J. Bacteriol. 182: 859-868.

Steel, R.G.D., Torrie, J.H. & Dickey, D.A. 1997. Principles and Procedures of Statistics, A Biometrical Approach. 3rd ed. New York: McGraw-Hill.

Sung, H., Ferlay, J., Siegel, R.L., Laversanne, M., Soerjomataram, I., Jemal, A. & Bray, F. 2021. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 71: 209-249.

Waks, A.G. & Winer, E.P. 2019. Breast cancer treatment: A review. JAMA 321: 288-300.

Zhang, T., Pan, D., Yang, Y., Jiang, X., Zhang, J., Zeng, X., Wu, Z., Sun, Y. & Guo, Y. 2020. Effect of Lactobacillus acidophilus CICC 6074 S-layer protein on colon cancer HT-29 cell proliferation and apoptosis. Journal of Agricultural and Food Chemistry 68(9): 2639-2647.

 

*Corresponding author; email: isnafia@apps.ipb.ac.id

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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